en Biotechnology Biotechnology كاربردي Orginal research papers An optimization procedure was applied to investigate the anticandidal property of four different Iranian seaweed extract compositions. Response Surface Methodology (RSM) with the four levels- three&ndash;factor Box&ndash;Behnken design, including <em>Gracilaria arquata </em>concentration (X₁)<em>, </em><em>Nizimudidnia zanardini </em>concentration (X₂)<em>, Cystoceria indica </em>concentration (X₃) and<em> Padina australis</em> concentration (X₄), was used to screen for <a>anticandidal </a>property screening. Multiple regression analysis of data showed that the coefficient determination (<em>R²</em>) was appropriate for the second-order model. The anticandidal property of the seaweed extract composition of 10 mg ml^-1<em> G. arquata</em> extract, 5 mg ml^-1<em>N. zanardini</em> extract, 4.5 mg ml^-1<em>C. indica</em> extract and 2 mg ml^-1<em>P. australis</em> extract was found to be optimal. Under the optimized composition, the <em>Candida albicans</em> growth inhibition zone was 26.23 mm. The experimental yield was well matched with the predicted yield. <div> <hr align="left" size="1" width="33%" > <div><div id="_com_1"></div></div> </div> Anticandidal property, Response Surface Methodology (RSM), Seaweed extract, Box-Behnken design, Candida albicans 881 892 http://jifro.ir/browse.php?a_code=A-10-367-2&slc_lang=en&sid=1 A. Taheri taherienator@gmail.com 100319475328460033949 100319475328460033949 Yes Fisheries Department, Faculty of marine sciences, Chabahar Maritime University, Chabahar, Iran, P.O.BOX. 99717- 56499.