en aquaculture aquaculture پژوهشي Orginal research papers Since chemotherapeutic agents used to control microbial fish diseases have led to resistance in bacteria, alternative methods have emerged in recent years. In this regard, identification of lactic acid bacteria with good probiotic properties and determination of their probiotic properties are extremely important. In this study, 25 lactic acid bacteria were isolated from freshwater fish and identified at species level using phenotypic, biochemical and molecular tests. The pH tolerance, antagonistic activity and antibiotic sensitivity of these 25 strains were examined, and they were considered as bacteria displaying the best activity in the potential probiotic treatment of fish diseases. <em>Lactococcus lactis</em> species numbered F2, F4, F9 and F10 were determined to have a potential probiotic capacity. Probiotic, Molecular identification, Lactic acid bacteria, ARDRA technique 1795 1807 http://jifro.ir/browse.php?a_code=A-10-1959-1&slc_lang=en&sid=1 Z. Hanol Bektas zubeydehanol17@gmail.com 100319475328460035546 100319475328460035546 Yes Aquaculture Research Station Directorate, Egirdir-ISPARTA/TURKEY F.B. Ucar 100319475328460035547 100319475328460035547 No Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir/TURKEY B. Giray 100319475328460035548 100319475328460035548 No Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir/TURKEY