en aquaculture aquaculture پژوهشي Orginal research papers <span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">A confirmatory method for the determination of </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">tetracycline antibiotics (TCs) </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">in Chinese mitten crab has been developed for the first time. The method comprises two stages: pretreatment of the samples and instrumental analysis. The samples are treated with Na2EDTA&ndash;McIlvaine buffer followed by extraction. The extract is then analyzed with ultra-performance liquid chromatography tandem high-resolution mass spectrometry (UPLC-HRMS). Four mostly used TCs, including </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">tetracycline</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">, oxytetracycline, chlortetracycline, and doxycycline, were detected in 10 min. The specificity was confirmed via the retention time and their high-resolution mass spectra. In the range of 50-500 &mu;g/kg, the linear regression coefficients (R</span></span></span>^{<span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">2</span></span></span>}<span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">) for all analytes were greater than 0.9985. Recoveries at three concentration levels were between 90.3% and 106.2%. The within-laboratory reproducibility was precise. The decision limit (CC_&alpha;) and detection capability (CC_&beta;) of </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">the method were determined using the permitted limit (100 &mu;g/kg) of TCs. The</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> detection results of TCs in 12 batches of Chinese mitten crab showed that no target residues were detected. This comprehensive method is compliant with the </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">2002/657/EC decision</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">. It could be applied for the determination of TCs in Chinese mitten crab samples and potentially in other aquatic products.</span></span></span> Tetracycline, Confirmatory method, Chinese mitten crab, UPLC, HR-MS 575 587 http://jifro.ir/browse.php?a_code=A-10-4920-1&slc_lang=en&sid=1 H. Wang 100319475328460052566 100319475328460052566 Yes Instrumental Analysis Center, Yancheng Teachers University, 224007, Yancheng, China H. Ding hui_0325@foxmail.com 100319475328460052567 100319475328460052567 No Yancheng Teachers University