fa پژوهشي Orginal research papers Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the <em>Novirhabdovirus </em>genus of the family &nbsp;<em>Rhabdoviridae.</em> This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (<em>Oncorhynchus mykiss)</em> samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in 2006. In addition, two VHSV strains were isolated from wild turbot (<em>Scophthalmus maximus</em>) in Trabzon Province of the Black Sea region of&nbsp; Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with 25 previously published gene sequences.&nbsp; Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-1.2 isolate&nbsp; (97.1-97.5% nucleotide identity and 98.2-98.4% amino acid identity) found in Georgia more than 30 years ago.&nbsp; These isolates could be&nbsp; an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have 97.5-97.6% nucleotide identity and 98.8-99% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey. Genotype, Rainbow trout, Turbot, Turkey, VHSV 67 73 http://jifro.ir/browse.php?a_code=A-10-1737-2&slc_lang=fa&sid=1 H. Albayrak harun.albayrak@omu.edu.tr 100319475328460029678 100319475328460029678 Yes Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey H. Isidan 100319475328460029679 100319475328460029679 No Department of Virology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey Department of Virology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey G. Kalayci 100319475328460029680 100319475328460029680 No Department of Virology, Bornova Veterinary Control Institute, İzmir, Turkey Department of Virology, Bornova Veterinary Control Institute, İzmir, Turkey E. Ozan 100319475328460029681 100319475328460029681 No Department of Virology, Veterinary Control Institute, Samsun, Turkey Department of Virology, Veterinary Control Institute, Samsun, Turkey V.N. Vakharia 100319475328460029682 100319475328460029682 No Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD, 21202, USA Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD, 21202, USA